The Cytonel Plus kit offers reagents for rapid pan-cytokeratin immunostaining, which is intended to assist the pathologist, who makes a rapid intraoperative histopathologic diagnosis using frozen sections. The kit enables immunostaining to be done in ~6 min.
Cytonel Plus kit content:
- Endogenous peroxidase blocker
- Peroxidased-conjugated Fab2 fragment of a pan-cytokeratin antibody, which is optimised for the ultrarapid staining protocol. The antibody is a cocktail of four mouse monoclonal antibodies. The antibody reacts with an epitope which is present in cytokeratins 4, 5, 6, 8, 10, 13, 18, and 19.
- Diamino benzidine (DAB) chromogen and dilution buffer
- Washing buffer (PBS-tween) concentrate
Kits for sized 20 and 100 slides are available.
Principle of the Cytonel Plus staining
See an example of Cytonel Plus stained sentinel node as a virtual slide (opens in new window)
Cytonel stain Cytonel stain
Brown-stained carcinoma cells are easily identifiable against counterstained (blue) normal lymphoid tissue.
Hematoxylin & eosin staining of the same lymph node (adjacent tissue section). Note difficulty to identify carcinoma cells (invasive lobular carcinoma).